《植物生理学报》 2012, 48(11): 1042-1049

欧石楠体细胞胚发生和植株再生

李际红, 邢世岩*, 姚培娟, 谭起航, 王海林

山东农业大学林学院, 农业生态与环境重点实验室, 山东泰安271018

通信作者：邢世岩；E-mail: xingsy@sdau.edu.cn；Tel: 0538-8243903

摘　要：

以欧石楠茎段为外植体, 研究其体细胞胚胎发生和植株再生。对影响茎段不定芽分化及胚性愈伤组织诱导的主导因 子进行比较分析, 并研究其体胚萌发、生根及移栽; 同时, 采用树脂切片法对茎段脱分化产生胚性愈伤组织及体胚发育过 程进行组织细胞学观察。结果表明, 接种在1/2WPM基本培养基上的茎段, 胚性愈伤组织诱导率为88.7%, 显著高于其他处 理, 不定芽诱导率可达90.6%, 平均分化倍数为3.6个, 平均分化苗高3.82 cm; 体细胞经过成熟培养后, 在添加1.0 mg•L-1 ZT和0.3 mg•L-1 IBA的1/2WPM培养基上萌发, 萌发的体胚在1/2WPM附加0.2 mg•L-1 NAA和0.3 mg•L-1 IBA的培养基上形成完整 的体胚苗植株, 体胚苗生根率达到87.4%, 经炼苗后移栽到蛭石:珍珠岩=3:1 (V/V)的栽培基质中, 成活率可达63.7%。在显微 镜下可观察到球形胚、心形胚、鱼雷形胚和子叶形胚; 体细胞胚以间接方式发生, 表现为愈伤组织外层细胞直接发生和愈 伤组织组织内部细胞发生。

关键词：欧石楠; 体细胞胚; 体胚发生; 植株再生; 组织细胞学观察

收稿：2012-07-18   修定：2012-10-18

资助：山东省泰安市科技局项目(2011-2013)和山东省泰安市大 学生科技引领创新计划项目(2008D2010和2009D1009)。

Somatic Embryogenesis and Plant Regeneration of Erica carnea L.

LI Ji-Hong, XING Shi-Yan*, YAO Pei-Juan, TAN Qi-Hang, WANG Hai-Lin

Key Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University, Tai’an, Shandong 271018, China

Corresponding author: XING Shi-Yan; E-mail: xingsy@sdau.edu.cn; Tel: 0538-8243903

Abstract:

In this study, we investigated somatic embryogenesis and plant regeneration in Erica carnea with stem section as explant. The effects on adventitious bud differentiation from stem and embryogenic callus induction were carried on comparative analysis. Subsequently, we explored its somatic embryo germination, rooting and transplantation. Meanwhile, embryogenic callus from stem differentiation and somatic embryogenesis were also observed by semi-section with resin embedded. The results showed that the induction rate (88.7%) of embryogenic callus on the basal medium 1/2WPM was higher than those on other media. The induction rate of adventitious bud, the average differentiation coefficient and the average height of differentiation seedlings reached 90.6%, 3.6 and 3.82 cm, respectively. Somatic cells germinated on the 1/2WPM medium with 1.0 mg·L^-1 ZT and 0.3 mg·L^-1 IBA after maturation. Thereafter, germinated somatic embryos formed complete plantlets, and rooting rate reached 87.4% on 1/2WPM medium containing 0.2 mg·L^-1 NAA and 0.3 mg·L^-1 IBA. The plantlets from somatic embryos were transplanted onto the substrate with vermiculite and perlite (3:1, V/V), and survival rate reached 63.7%. Furthermore, globular embryos, heart-shaped embryos, torpedo embryos and cotyledon embryos were observed under a microscope. The somatic embryogenesis was occurred in two ways: direct somatic embryogenesis from outer cells of callus and indirect somatic embryogenesis from intercells of callus.

Key words: Erica carnea; somatic embryo; somatic embryogenesis; plant regeneration; cytohistological observation